DNA, from homogeneous powder of ground rice grains, rice
flour and rice noodles, was extracted using a CTAB-based procedure
(ISO 21571), in combination with the Genomic-tip20/G,
which was adapted from the EU-RL GMFF (European Union
Reference Laboratory for GM Food and Feed) validated method
(European Union Reference Laboratory., 2006; International
Standard ISO 21571, 2005). DNA concentration was measured by
spectrophotometry using the Nanodrop 2000 device and DNA
purity was evaluated as falling within the acceptance criteria
according to the A260/A280 (1.8) and A260/A230 (2.0–2.2)
ratios. DNA extraction, concentration and purity of WT maize were
carried out as previously described (Broeders et al., 2012c).