This research studied the fermentation profile of fermented Queen pineapple juice with yeast and lactic acid bacteria starter cultures. Sequential fermentations with yeast starter culture of Saccharomyces cerevisiae and Saccharomycodes lidwigii, LAB starter culture of Lactobacillus plantarum and Oenococcus oeni, and Mixed fermentations with mixed starter culture of S. cerevisiae and L. plantarum, S. cerevisiae and O. oeni, S. ludwigii and L. plantarum, S. ludwigii and O. oeni.