2.1. Microorganisms
The used fungi for the production of chitosan in this study was Cunninghamella echinulata var. elegans, deposited as C. elegans (RCMB-012002), and was obtained from the Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt.
E. coli (ATCC-25922), Staphylococcus aureus (ATCC-25923), Can- dida albicans (ATCC-10231) and Penicillium expansum (RCMB-1006) were used for the antimicrobial experiments.