The intracellular polysaccharides (CLSP) were extracted from Lepista sordida mycelium in submerged
culture followed by concentration and ethanol precipitation. The antioxidant activities of CLSP were
evaluated both in vitro and in vivo. In vitro antioxidant assay, CLSP had noticeable scavenging activities
on superoxide anion, hydroxyl radical and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical. For antioxidant
testing in vivo, different doses of CLSP were orally administrated over a period of 6 weeks in a d-galactose
induced aged mice model. As results, CLSP significantly inhibited the formation of malondialdehyde
(MDA) and raised the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in
mice brains and serums in a dose-dependent manner. The results provide a reference for large-scale
production of CLSP by submerged fermentation and suggested that CLSP had potent antioxidant activity
and could be explored as a potential dietary supplement to retard aging and attenuate age-related diseases
in humans.