Magnetic resonance imaging (MRI) was used to monitor internal changes in harvested tomato (Solanumlycopersicum L. cv. Micro-Tom) fruit. Measurements of ethylene evolution, respiration, and ion leakageindicated that the fruit developed chilling injury (CI) after storage at 0◦C. Unlike these measurements,MRI provided spatially resolved data. The apparent diffusion coefficient (ADC), which is an indication ofwater mobility in tissues, was calculated from MRIs of the different parts of the fruit. Storage for 1 or 2weeks at 0◦C caused no difference in the ADCs (D-values) in the pericarp, but it did lead to higher values inthe inner tissues i.e., the columella and locular region compared to non-chilled fruit (P < 0.05). Changes ininner fruit D-values after 1 and 2 weeks of chilling at 0◦C were similar to changes in respiration, ethyleneproduction and ion leakage which increased (P < 0.05) compared to the non-chilled controls. Most CIstudies of tomato fruit used pericarp tissue. Our data indicate that columella tissue changes occur inresponse to chilling injury in tomato fruit and suggest that more caution is needed when interpretingdata from experiments commonly used to study this phenomenon
Magnetic resonance imaging (MRI) was used to monitor internal changes in harvested tomato (Solanumlycopersicum L. cv. Micro-Tom) fruit. Measurements of ethylene evolution, respiration, and ion leakageindicated that the fruit developed chilling injury (CI) after storage at 0◦C. Unlike these measurements,MRI provided spatially resolved data. The apparent diffusion coefficient (ADC), which is an indication ofwater mobility in tissues, was calculated from MRIs of the different parts of the fruit. Storage for 1 or 2weeks at 0◦C caused no difference in the ADCs (D-values) in the pericarp, but it did lead to higher values inthe inner tissues i.e., the columella and locular region compared to non-chilled fruit (P < 0.05). Changes ininner fruit D-values after 1 and 2 weeks of chilling at 0◦C were similar to changes in respiration, ethyleneproduction and ion leakage which increased (P < 0.05) compared to the non-chilled controls. Most CIstudies of tomato fruit used pericarp tissue. Our data indicate that columella tissue changes occur inresponse to chilling injury in tomato fruit and suggest that more caution is needed when interpretingdata from experiments commonly used to study this phenomenon
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