photosynthesis and heterotrophic growth occur simultaneously
and independently in mixotrophic cultures (Marquez et al.,
1993), the presence of organic carbon can alter both the photosynthetic
and heterotrophic metabolism of Chlorella (Villarejo et al.,
1995). The lipid and biomass production of mixotrophic cultivation
are much higher than under autotrophic and heterotrophic cultivation
indicated in previous work (Table 1). Thus, these findings indicate
that adding organic carbon sources leads to a marked
stimulation of lipid accumulation in Chlorella sp. Y8-1, and that
the ideal organic carbon source for the production of lipids is
sucrose