The Sm and the Vs strains result from degradation of the L strain usually because the
culture has become old. Degeneration can be accelerated by bad conservation techniques.
Xanthan gum is produced using always the L strain and a good conservation of the strain is
necessary. Different techniques have been devised for short- and long-term conservation of
the microorganism (Jeanes et al., 1976), as follows: Long-term conservation is a nonpropagative
conservation technique that uses lyophylization and freezing in 10% (v/v)glycerin solutions. The short-term conservation methods allow some microbial growth. The
cells are grown on complex solid media (e.g. YM agar) slants and plates for 18±20 h at 25°C
(Cadmus et al., 1976). The slants and plates are then maintained at 4°C. The culture must be
transferred to fresh medium every 14 days to prevent strain degradation (Silman and
Rogovin, 1970; Cadmus et al., 1976; De Vuyst et al., 1987a,b). For checking the culture
viability, the YM agar slant is incubated at 25°C for 3 days; vigorous cells produce bright
yellow and round colonies of 4±5 mm in diameter.
The Sm and the Vs strains result from degradation of the L strain usually because theculture has become old. Degeneration can be accelerated by bad conservation techniques.Xanthan gum is produced using always the L strain and a good conservation of the strain isnecessary. Different techniques have been devised for short- and long-term conservation ofthe microorganism (Jeanes et al., 1976), as follows: Long-term conservation is a nonpropagativeconservation technique that uses lyophylization and freezing in 10% (v/v)glycerin solutions. The short-term conservation methods allow some microbial growth. Thecells are grown on complex solid media (e.g. YM agar) slants and plates for 18±20 h at 25°C(Cadmus et al., 1976). The slants and plates are then maintained at 4°C. The culture must betransferred to fresh medium every 14 days to prevent strain degradation (Silman andRogovin, 1970; Cadmus et al., 1976; De Vuyst et al., 1987a,b). For checking the cultureviability, the YM agar slant is incubated at 25°C for 3 days; vigorous cells produce brightyellow and round colonies of 4±5 mm in diameter.
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