At present, Thai people consume more bakery products due to the hustle of everyday life and the culture was changed. Which the tropical climate of Thailand is not conducive to keeping fresh food for a long time. Thus causing many traders to choose preservatives to extend the shelf life of food. Without taking into account the long-term health effects that may occur to consumers. The purpose of this research is to develop a package of microorganism inhibitors. For contain in packaging with bakery products. By using extracts from natural essential oils mixed with Polybutylene Succinate (PBS) by means of encapsulation method to obtain an antimicrobial beads. Therefrom packed into sachet control release that coat with Ethylene-vinyl alcohol (EVOH). After packed antimicrobial sachet in bread packaging, it was not found fungus on bread after expired date more than 2 months