Fig. 1: Pictorial overview of isolation of Sulfolobus viruses. (A) Wolfram Zillig sampling at a typical Sulfolobus-containing pool in Yellowstone National
Park, USA, September 2000 (inset shows anaerobic tubes with samples). (B) 80°C incubator with long-necked growth flasks (detail in inset). (C) Singlecolony
isolates of Sulfolobus solfataricus on a Gelrite® plate. This plate contains a mixture of S. solfataricus containing (blue colonies) and lacking (brown)
a vector expressing the lacS gene from S. solfataricus and was sprayed with 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-gal) (see Jonuscheit et
al. 2003). (D) Lawn of S.solfataricus strain P1 with halos of growth inhibition due to virus production by 2-μl spots of virus-infected strains. Spots labeled
SV2P1 and SV2P2 are from S. solfataricus strains P1 and P2 infected with SSV-I2 respectively (Stedman et al. 2003). Spot labeled C is a detergent-positive
control. Spot labeled P2- is uninfected S. solfataricus strain P2 as a negative control.