In the SSF process, cellulose of sugarcane bagasse was converted into glucose by cellulose enzymes then glucose was converted into ethanol by Saccharomyces cerevisiae simultaneously. The GC charts of ethanol content as the SSF results was shown in Figure 2. It can be seen that the ethanol peak appears at about 2.5 min to 2.7 min. The peak appears at about 2.7 min to 2.9 min is propanol peak, which is used as control in the analysis. The ethanol dry weight conversion results of three incubation time variables was described in a graph, which is shown in Figure 3. Figure 3 shows that the incubation time variables have maximum ethanol content between 4 d to 5 d incubation time.