This paperpresentsquantificationoffiveanthraquinonederivatives(emodinglycoside,chrysophanol
glycoside,emodin,chrysophanolandphyscion)inrhizomesofhardenedmicro-propagated Rheum
emodi plantsusinghigh-performanceliquidchromatography(HPLC).Asepticshootcultureswereraised
using rhizomebuds.ShootmultiplicationoccurredinbothagargelledandliquidMurashigeandSkoog
(MS) mediumsupplementedwith10.0 mM 6-benzylaminopurine(BAP)and5.0 mM indole-3-butyric
acid (IBA).Rootedplantletsobtainedonplantgrowthregulator(PGR)-freemediumweretransferredto
soil with92%survival.HPLCanalysisrevealedthepresenceoffiveanthraquinonederivatives:emodin
glycoside,chrysophanolglycoside,emodin,chrysophanolandphyscioninrhizomesoftissueculture-
raised plants.Onlyemodinglycoside(1)andchrysophanolglycoside(2)werepresentin6-month-old
hardenedtissueculturedplants.Inaddition,theotherthreederivatives(emodin(3),chrysophanol(4)
and physcion(5))werealsodetectedafter9months.
Paperpresentsquantificationoffiveanthraquinonederivatives นี้ (emodinglycoside, chrysophanol
glycoside, emodin, chrysophanolandphyscion) เผยแพร่ inrhizomesofhardenedmicro Rheum
emodi plantsusinghigh-performanceliquidchromatography(HPLC)Asepticshootcultureswereraised
rhizomebuds ใช้ShootmultiplicationoccurredinbothagargelledandliquidMurashigeandSkoog
(MS) mediumsupplementedwith10มม. 0 6-benzylaminopurine (BAP) เวย์ and5.0 มม.อินโดล-3-butyric
กรด (อิบา)Rootedplantletsobtainedonplantgrowthregulator (PGR) -freemediumweretransferredto
ดิน with92% อยู่รอดHPLCanalysisrevealedthepresenceoffiveanthraquinonederivatives:emodin
glycoside, chrysophanolglycoside, emodin, chrysophanolandphyscioninrhizomesoftissueculture-
ยกพืชOnlyemodinglycoside (1) andchrysophanolglycoside (2) werepresentin6-เดือนอายุ
hardenedtissueculturedplantsInaddition, theotherthreederivatives (emodin (3) chrysophanol (4)
และ physcion(5)) werealsodetectedafter9months
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นี้ paperpresentsquantificationoffiveanthraquinonederivatives(emodinglycoside,chrysophanol
glycoside,emodin,chrysophanolandphyscion) โกฐน้ำเต้า
emodi plantsusinghigh - performanceliquidchromatography ( hplc ) asepticshootcultureswereraised
การใช้ rhizomebuds . shootmultiplicationoccurredinbothagargelledandliquidmurashigeandskoog
(มิลลิวินาที) mediumsupplementedwith10 .0 มม. 6 - benzylaminopurine (, BAP )และ indole 5.0 มม. - 3 - butyric
กรด(กลุ่มบริการด้าน) rootedplantletsobtainedonplantgrowthregulator ( pgr ) - freemediumweretransferredto
ดิน with92%survival.HPLCanalysisrevealedthepresenceoffiveanthraquinonederivatives:emodin
glycoside,chrysophanolglycoside,emodin,chry พันธุ์ไม้ต่างๆ.Onlyemodinglycoside(1)andchrysophanolglycoside(2)werepresentin6-month-old
hardenedtissueculturedplants.Inaddition,theotherthreed physcion ( 5 )) werealsodetectedafter 9 เดือน.
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