Furthermore, macroalgae integrated culture during 120 days of shrimp growth period (Experiment 2, Fig. 4), exhibited the similar trend of nutrient removal and the differences now was the initial concentration of ammonium was in the range of 5.5 e 6.5g m3.This value is three times higher than it was in Experiment 1. G. edulis illustrated 60% reduction (F ¼ 235.1, P < 0.01) and U. lactuca was 50% (F ¼ 44.3, P < 0.01). Similarly, both these macroalgae species significantly absorbs ammonium as in Experiment 1. Without macroalgae, control tank, has no significant nutrient removaland recorded F probability value of 13.44 (P > 0.05).