Total anthocyanins and polyphenols determinations
The total anthocyanins were determined by a modified pH-differential method, as reported by Alper et
al (1996) [4]. The spectrophotometric measurements were carried out using a UV-Vis spectrophotometer
(Jasco Europe) and all the absorbance readings were made against distilled water as a blank.
The Folin–Ciocalteu assay method was used for the determination of the total phenol content in the
samples, colourimetrically measured and expressed as Gallic acid equivalents.
The analytical determinations were carried out in triplicate. Analysis of variance (ANOVA) was used
to evaluate the repeatability of the experimental data. Student tests were used as paired comparisons
between sample means. The level of significance was set to 0.05.