Whole rice samples were ground in a cyclone mill (Cyclotec
1093; Foss Tecator, Hamburg, Germany) fitted with a 0.5 mmsieve.
Lipid extracts were extracted with Et2O for 12 h from the samples
using a Soxhlet apparatus. The solvent was then removed in a
rotary evaporator at 40 C, with the extracted lipid extracts dissolved
in CHCl3 and stored at 80 C until used. Extractions and
analyses were performed in duplicate.