The total phenolic content was determined by the Folin-
Ciocalteu assay as described by Singleton, Orthofer, and Lamuela-
Raventos (1999) and expressed as mg of gallic acid equivalent
(GAE) per kg of dry matter (d.m.). One hundred mL of the extract
was transferred into test tubes and their volume made up to 500 mL
with distilled water. After addition of Folin-Ciocalteu reagent