The cytotoxic effect and reactive oxygen species formation were
examined only for plain blackberry leaf extract in order to provide
insight in the biological activity of this medicinal plant. As can be
seen in Fig. 2a, in the case of 1 h treatment with the recovery period
of 24 h, the cytotoxic effect of blackberry leaf extract occurs at
the concentration of 0.75 and continues to grow as a function of
extract concentration. Cytotoxic effect of blackberry extract was
determined in the case when cells were left to recover for 24 h
after the treatment; in the case of 2 h treatment with the blackberry
leaf extract, the cytotoxic effect is detected at concentrations
higher than 1. It can be concluded that cytotoxic processes in the
cell are triggered after removal of blackberry extract and during
recovery period of 24 h. This indicates that cytotoxic effect of this
extract cannot be determined immediately after the treatment.
Also, the obtained results indicate that the cytotoxic effect of
blackberry leaf extract depends on its concentration. S