Computational Analysis of Genes Induced in Olive Roots
by P. Fluorescens PICF7
Analysis of the EST set by the Blast2GO software allowed the
annotation of expressed sequences according to the terms of the
three main GO vocabularies, i.e. ‘biological process’, ‘molecular
function’ and ‘cellular component’. It is worth mentioning that
only 76.6% of the sequences were mapped by the GO annotation
database. Thus, 104 ESTs (85 unknown and 19 predicted
sequences corresponding to the remaining 23.4%) were not
included in this functional classification because they were
automatically discarded by the program before running the
analysis. Since some translated sequences were identified by
different GO terms, they were classified according to the best hits
retrieved from the BLAST homology search. ESTs distribution for
‘biological processes’, ‘molecular function’ and ‘cellular component’
main categories are shown in Figure 1.
Regarding to biological processes, 56% (190 sequences) of the
transcripts corresponded to very low represented GO terms
categories (i.e., cell cycle, flower and reproductive structures
development, cellular homeostasis, photosynthesis, pollination, cell
differentiation, embryonic and post-embryonic development), and
were grouped as ‘other’ in our analysis (Fig. 1A). Concerning to