This system helps to organize a workplace for efficiency and decrease wasting and optimize quality and productivity via monitoring an organized environment. It also provides useful visual evidences to obtain more firm results. There is a real need for empirical studies in field of new management systems and their impact on company’s performance. As importance role of continuous improvement in today’s organizations, and lack of sufficient evidence to show the positive impact of 5S on organizational performance, this paper aims to determine performance factors and characteristics in industrial organizations and identifying the effectiveness of 5S implementation on organizational performance as well. Surveying method is used and data collection is done by distributing questionnaire among five target organizations which have implemented 5S techniques. The target organizations are chosen from different industries and diverse field of work. The results of this research obtained from a comparative measurement of organizational performance before and after 5S implementation. The results show that 5S is an effective tool for improvement of organizational performance, regardless of organization type, size, its production or its service. Consequently, 5S techniques would strongly support the objectives of organization to achieve continuous improvement and higher performance. Keywords: 5S, performance, total quality management, productivity