In freshly matured seeds, two small first true leaves 142.4 ± 16.9 mm in length were observed on the epicotyl plumule
(Fig. 3a), and they grew to a length of 430.9 ± 24.5 mm during 12 weeks of cold stratification (Fig. 3b). Many small lipid bodies and some large protein bodies and vacuoles were observed in all cells in the plumule apical meristem of fresh seeds (Fig. 3c). The number of lipid and protein bodies was reduced greatly during cold stratification
for 12 weeks, and cell division resulted in formation of new cells (Fig. 3d). Many small lipid bodies and some vacuoles, but no protein bodies, were observed in cells of the first true leaves of fresh seeds (Fig. 3e), and the lipid bodies decreased greatly in number during cold stratification for 12 weeks (Fig. 3f). Cells in the cotyledons of fresh seeds contained many lipid bodies and one large protein body (Fig. 3g), and a large central vacuole was observed during cold stratification, and it continued to decrease when the radicle emerged. Myo-inositol, glucose and mannose decreased
about 50% during cold stratification; however, they rapidly increased three-to four-fold in radicle-emerged seeds. Raffinose remained at the same level in fresh and cold-stratified seeds but decreased about seven-fold as the radicle emerged. The concentration of melibiose was 44 mg (g DW)1 in fresh seeds, and it was significantly reduced to 23e26 mg (g DW)1 in cold-stratified and radicle-emerged seeds. The concentration of trehalose remained at about the same level, 44 mg (g DW)1, in fresh, cold-stratified and radicle-emerged seeds (Table 5).
In freshly matured seeds, two small first true leaves 142.4 ± 16.9 mm in length were observed on the epicotyl plumule(Fig. 3a), and they grew to a length of 430.9 ± 24.5 mm during 12 weeks of cold stratification (Fig. 3b). Many small lipid bodies and some large protein bodies and vacuoles were observed in all cells in the plumule apical meristem of fresh seeds (Fig. 3c). The number of lipid and protein bodies was reduced greatly during cold stratificationfor 12 weeks, and cell division resulted in formation of new cells (Fig. 3d). Many small lipid bodies and some vacuoles, but no protein bodies, were observed in cells of the first true leaves of fresh seeds (Fig. 3e), and the lipid bodies decreased greatly in number during cold stratification for 12 weeks (Fig. 3f). Cells in the cotyledons of fresh seeds contained many lipid bodies and one large protein body (Fig. 3g), and a large central vacuole was observed during cold stratification, and it continued to decrease when the radicle emerged. Myo-inositol, glucose and mannose decreasedabout 50% during cold stratification; however, they rapidly increased three-to four-fold in radicle-emerged seeds. Raffinose remained at the same level in fresh and cold-stratified seeds but decreased about seven-fold as the radicle emerged. The concentration of melibiose was 44 mg (g DW)1 in fresh seeds, and it was significantly reduced to 23e26 mg (g DW)1 in cold-stratified and radicle-emerged seeds. The concentration of trehalose remained at about the same level, 44 mg (g DW)1, in fresh, cold-stratified and radicle-emerged seeds (Table 5).
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