Experimental animals, rearing conditions and feeding trial
The larvae used in this study were obtained from wild spawners
caught from Miyasaki prefecture, Japan. The trial was run using
zoea stage larvae (Z1). Groups of 100 Z1 were randomly stocked
into 12 1-L beakers containing 750 ml of 34 ppt, membrane filtered
(50μm) and UV sterilized seawater. Aeration for each beaker was
supplied through a glass Pasteur pipette with tip reaching to the
bottom, providing a mild stream of air that circulated water inside
the container, keeping feed particles in suspension.