In order to obtain the optimal condition of ultrasonic cell-break method for coenzyme Q10 extraction, the effect of output power, duration of radiation each time, total working time and amount of water on cell-wall disruption and coenzyme Q10 extraction were systematically studied in this paper. The results showed that the optimal condition for coenzyme Q10 extraction was as follows output power 500 W, radiation/intermission time 12 s/10 s, total working time 12 min, and amount of water 45 mL/g; the quantity of coenzyme Q10 extracted by ultrasonic cell-break method could reach 1.196 mg/g under the optimal condition. It’s proved that the method of the ultrasonic cell-break for extracting coenzyme Q10 was feasible and more effective than the method of non-broken, grinding, freezing and melting repeatedly.