The Cry1Ab gene transcript levels were detected by RT-PCR using the primers 5-caccacagaacaacaatgtgccacc-3 and 5ctgtctagagtactcagcctcgaaggtaa-3,resultingina604bpfragment, under the following PCR conditions: 95◦C for 5min, followed by 32 cycles of 95◦C for 30s, 58◦C for 30s and extension at 72◦C for 30s, and a final extension at 72◦C for 10min. The rice (Oryza sativaL.)ubiquitin1(Ubi-1)genewasusedasaninternalcontrol.42