Haematococcus pluvialis Flotow K-0084 was obtained from
the Scandinavian Culture Collection of Algae and Protozoa
at the University of Copenhagen, Denmark and maintained
in BG11 culture medium (Boussiba and Vonshak 1991). In
the green stage, H. pluvialis was cultivated in a glass column
(inner diameter 5 cm; working volume 0.6 L) under
indoor conditions (20 °C, 20 μmol photons m−2 s
−1 of
continuous light)