4. Add 400 µl melted 1% SeaKem Gold agarose to 400 µl cell suspension; mix by gently pipetting mixture up and down a few times. Over-pipetting can cause DNA shearing. Maintain temperature of melted agarose by keeping flask in beaker of warm water (55-60°C).
5. Immediately, dispense part of mixture into appropriate well(s) of reusable plug mold. Do not allow bubbles to form. Two plugs of each sample can be made from these amounts of cell suspension and agarose and are useful if repeat testing is required. Allow plugs to solidify at room temperature for 10-15 minutes. They can also be placed in the refrigerator (4°C) for 5 minutes