3.1. Collect of efficient liquid enzyme
P. pastoris is frequently used as a host for expression of heterologous proteins because it can be tightly regulated by a eukaryotic promoter (alcohol oxidase I; AOX1) and
can tolerate a broad pH range (3.0–7.0) (Soyaslan and Calik, 2011). To date, 500 heterologous
proteins have been expressed in P. pastoris systems (Yu et al.,
2010).