Establishment of optimal g-irradiation dose and radioprotective effect of the aqueous extract of C. longa
on rat hepatocytes Before studying the effect of g-irradiation on primary rat hepatocytes, we determined the cell viability at different gradiation doses (0–8 Gy). The dose–response curve for rat hepatocytes indicated that 3.2 Gy was the 50% inhibitory
dose (ID50). This dose was selected for exposing hepatocytes to g-irradiation (Fig. 1a).