Theintroductionofon-linesolid-phase

Theintroductionofon-linesolid-phaseextraction(SPE) inHPLC-NMRhasdramaticallyenhancedthesensitivity ofthistechniquebyconcentrationoftheanalytesina small-volumeNMRflowcellandbyincreasingtheamount oftheanalytebymultiplepeaktrapping.Inthisstudy, thepotentialofHPLC-DAD-SPE-NMRhyphenationwas demonstratedbystructuredeterminationofcomplex constituentsofflower,leaf,root,andstemextractsofan AfricanmedicinalplantKanahialaniflora.Thetechniquewasshowntoallowacquisitionofhigh-quality homo-andheteronuclear2DNMRdatafollowinganalytical-scaleHPLCseparationofextractconstituents.Four flavonolglycosides[kaempferol3-O-(6-O-r-L-rhamnopyranosyl)-â-D-glucopyranoside;kaempferol3-O-(2,6-di-Or-L-rhamnopyranosyl)-â-D-glucopyranoside;quercetin3-O(2,6-di-O-r-L-rhamnopyranosyl)-â-D-glucopyranoside(rutin); andisorhamnetin,3-O-(6-O-r-L-rhamnopyranosyl)-â-Dglucopyranoside]andthree5r-cardenolides[coroglaucigenin3-O-6-deoxy-â-D-allopyranoside;coroglaucigenin3-O(4-O-â-D-glucopyranosyl)-6-deoxy-â-D-glucopyranoside;3′O-acetyl-3′-epiafroside]wereidentified,withcomplete assignmentsof1Hand13CresonancesbasedonHSQC andHMBCspectrawheneverrequired.Confirmationof thestructureswasprovidedbyHPLC-MSdata.The HPLC-DAD-SPE-NMRtechniquethereforespeedsupthe dereplicationofcomplexmixturesofnaturaloriginsignificantly,bycharacterizationofindividualextractcomponentspriortopreparativeisolationwork

ผลลัพธ์ (ไทย) 1:

Theintroductionofon-linesolid-phaseextraction(SPE) inHPLC NMRhasdramaticallyenhancedthesensitivity ofthistechniquebyconcentrationoftheanalytesina oftheanalytebymultiplepeaktrapping volumeNMRflowcellandbyincreasingtheamount เล็กInthisstudy, demonstratedbystructuredeterminationofcomplex thepotentialofHPLC-พ่อ-SPE-NMRhyphenationwas constituentsofflower ใบ รากandstemextractsofan AfricanmedicinalplantKanahialaniflora.Thetechniquewasshowntoallowacquisitionofhigh คุณภาพตุ๊ด-andheteronuclear2DNMRdatafollowinganalytical-scaleHPLCseparationofextractconstituents.Four flavonolglycosides[kaempferol3-O-(6-O-r-L-rhamnopyranosyl)-â-D-glucopyranoside;kaempferol3-O-(2,6-di-Or-L-rhamnopyranosyl)-â-D-glucopyranoside;quercetin3-O(26-di-O-r-L-rhamnopyranosyl)-â-D-glucopyranoside(rutin) andisorhamnetin,3-O-(6-O-r-L-rhamnopyranosyl)-â-Dglucopyranoside]andthree5r-cardenolides[coroglaucigenin3-O-6-deoxy-â-D-allopyranoside;coroglaucigenin3-O(4-O-â-D-glucopyranosyl)-6-deoxy-â-D-glucopyranoside;3′O-acetyl-3′-epiafroside]wereidentified,withcomplete assignmentsof1Hand13CresonancesbasedonHSQC andHMBCspectrawheneverrequired.Confirmationof thestructureswasprovidedbyHPLC-MSdata.The HPLC-พ่อ-SPE-NMRtechniquethereforespeedsupthe dereplicationofcomplexmixturesofnaturaloriginsignificantly, bycharacterizationofindividualextractcomponentspriortopreparativeisolationwork

ผลลัพธ์ (ไทย) 2:

Theintroductionofon-linesolid-phaseextraction (SPE) inHPLC-NMRhasdramaticallyenhancedthesensitivity ofthistechniquebyconcentrationoftheanalytesina เล็ก volumeNMRflowcellandbyincreasingtheamount oftheanalytebymultiplepeaktrapping.Inthisstudy, thepotentialofHPLC-DAD-SPE-NMRhyphenationwas constituentsofflower demonstratedbystructuredeterminationofcomplex ใบราก andstemextractsofan AfricanmedicinalplantKanahialaniflora.Thetechniquewasshowntoallowacquisitionofhigh คุณภาพตุ๊ด andheteronuclear2DNMRdatafollowinganalytical-scaleHPLCseparationofextractconstituents.Four assignmentsof1Hand13CresonancesbasedonHSQC andHMBCspectrawheneverrequired.Confirmationof thestructureswasprovidedbyHPLC-MSdata.The HPLC-DAD-SPE-NMRtechniquethereforespeedsupthe

ผลลัพธ์ (ไทย) 3:

theintroductionofon linesolid phaseextraction ( SPE ) inhplc nmrhasdramaticallyenhancedthesensitivity ofthistechniquebyconcentrationoftheanalytesina ขนาดเล็ก volumenmrflowcellandbyincreasingtheamount oftheanalytebymultiplepeaktrapping . inthisstudy thepotentialofhplc พ่อ , เอสพี nmrhyphenationwas demonstratedbystructuredeterminationofcomplex constituentsofflower ใบ รากandstemextractsofan africanmedicinalplantkanahialaniflora.thetechniquewasshowntoallowacquisitionofhigh-quality homo-andheteronuclear2dnmrdatafollowinganalytical-scalehplcseparationofextractconstituents.four flavonolglycosides [ kaempferol3-o - ( 6-o-r-l-rhamnopyranosyl ) - â - D-glucopyranoside ; kaempferol3-o - ( 2,6-di-or-l-rhamnopyranosyl ) - â - D-glucopyranoside ; quercetin3-o ( 26-di-o-r-l-rhamnopyranosyl ) - â - D-glucopyranoside ( สัตว์ ) ; andisorhamnetin 3-o , - ( 6-o-r-l-rhamnopyranosyl ) - â - dglucopyranoside ] andthree5r คาร์ดิโนไลด์ [ coroglaucigenin3-o-6-deoxy - â - d-allopyranoside ; coroglaucigenin3-o ( 4-o - â - d-glucopyranosyl ) - 6-deoxy - â - D-glucopyranoside 3 นั้นได้รับ o-acetyl-3 - epiafroside ] wereidentified withcomplete assignmentsof1hand13cresonancesbasedonhsqc , andhmbcspectrawheneverrequired .confirmationof thestructureswasprovidedbyhplc-msdata.the HPLC พ่อเอสพี nmrtechniquethereforespeedsupthe dereplicationofcomplexmixturesofnaturaloriginsignificantly bycharacterizationofindividualextractcomponentspriortopreparativeisolationwork ,