Lipid content was measured by the acid hydrolysis method. The MLE (1 g) were mixed with hydrochloric acid (20 ml) in
a conical flask, then heated in a water bath at 70–80 C for about 50 min. After heating, the mixture was cooled to
room temperature, and then 10 mL of ethanol and 20 ml of ethyl ether were added to a separatory funnel. The separatory funnel was vigorously shaken to ensure complete mixing of the two liquid phases. Then, the two liquid
phases were allowed to separate for at least 2 h until the layers were clearly separated. The lower solvent was collected in a new beaker, and the upper solvent (ethyl ether) was collected in a conical flask. Ethyl ether (20 ml) was
added to the lower solvent and poured into a separatory funnel, which was shaken vigorously to ensure complete mixing of the two liquid phases. The above process was repeated three times. The collected ethyl ether was evaporated under vacuum and then dried in an oven. The weight of the flask was measured and used as the content
of lipid after the flask tare weight was subtracted
Lipid content was measured by the acid hydrolysis method. The MLE (1 g) were mixed with hydrochloric acid (20 ml) in
a conical flask, then heated in a water bath at 70–80 C for about 50 min. After heating, the mixture was cooled to
room temperature, and then 10 mL of ethanol and 20 ml of ethyl ether were added to a separatory funnel. The separatory funnel was vigorously shaken to ensure complete mixing of the two liquid phases. Then, the two liquid
phases were allowed to separate for at least 2 h until the layers were clearly separated. The lower solvent was collected in a new beaker, and the upper solvent (ethyl ether) was collected in a conical flask. Ethyl ether (20 ml) was
added to the lower solvent and poured into a separatory funnel, which was shaken vigorously to ensure complete mixing of the two liquid phases. The above process was repeated three times. The collected ethyl ether was evaporated under vacuum and then dried in an oven. The weight of the flask was measured and used as the content
of lipid after the flask tare weight was subtracted
การแปล กรุณารอสักครู่..
![](//thimg.ilovetranslation.com/pic/loading_3.gif?v=b9814dd30c1d7c59_8619)