Estimation of striatal oxidative stress biomarkers
MDA level
Striatal MDA level was estimated using the Biodiagnostic colorimetric kit (Cairo, Egypt) according to the method described by Ohkawa et al. (1979). MDA forms a pink-colored complex with thiobarbituric acid, in acidic medium at 95 °C for 30 min. The absorbance was measured at 534 nm and the results were expressed as nmol/mg protein.