Nauplii were collected from 500 l intensive cultures and placed in clean diluted
seawater Ž18‰. for 24 h. Approximately 17% of the nauplii were collected for
immediate fatty acid analysis. A further 17% of the nauplii were placed in a separate
container and provided a combination of I. galbana and Nannochloropsis oculata
ŽCSIRO culture code CS-179. at densities of 5=105 and 3=106 cellsrml, respectively.
Remaining nauplii were fed I. galbana at the rate of 5=105 cellsrml. In each
treatment, nauplii were stocked at approximately 200rml and subjected to gentle
aeration at 238C. Nauplii provided with I. galbana and N. oculata were collected for
fatty acid analysis after 6 h. Those provided I. galbana were collected for analysis after
0.5, 2, 4 and 6 h.