The DNA standard ranging from 2.88 × 107copies/l to2.88 × 100copies/l was tested repeatedly. Three separate dilu-tion series were assayed in a single run to evaluate intra-assay variations. Whereas, the inter-assay variations were measured by testing each dilution in three separate consecutive runs. The mean,standard deviation (SD) and coefficient of variation (CV) for both intra-assay and inter-assay variations were calculated separately for each standard DNA dilution based on their Ct values using Microsoft Excel software.