Rice bran was donated by a local mill in Surabaya (Indonesia)
and was kept at 60 ◦C in oven for a month to increase its free
fatty acid (FFA) contents and to reduce its water content. Afterwards,
the bran was stored at −60 ◦C to prevent the formation
of free fatty acid caused by the hydrolysis of acylglycerols catalyzed
by lipase contained in rice bran. Thin-layer chromatograph
(TLC) aluminum plates (20cm×20cm×250m) were purchased
from Merck (Darmstadt, Germany). Triacylglycerols were obtained
from commercial sources. Standard fatty acids were obtained from
Brataco Chemika Company (Jakarta, Indonesia). All solvents and
reagents were either of high-performance liquid chromatography
(HPLC) grade or analytical reagent grade and were obtained from
commercial sources.