2.6. In-direct FT-RIS measurements
The experimental setup for the optical measurements on the
microfluidic sensing device was illustrated in Scheme 1b. The re-
flectance spectra of each test zone on the chip were acquired by
Ocean Optics USB2000þ spectrometer which was fitted to a microscopic
lens via fiber optics. White light from a tungsten lamp
(Ocean Optics) was fed through one arm of a bifurcated fiber optic
cable and focused through a lens onto the test zone chip at normal
incidence. Reflected light was collected through the same optics,
and data was analyzed with Spectrasuit software (Ocean Optics
Inc.) as described previously (Pacholski et al., 2005). The effective
optical thickness (EOT) was calculated with the Fabry-Pérot relationship: