4) Using one of the droppers supplied in the kit, add 25 L(1 drop of 25 uL) of Control Cells to well#1. Using another dropper supplied in the kit, 25 puL(1 drop of 25 uL) of Antigen-Sensitized Cells to wells#2 through#8(or more)
5) Mix the content of the wells thoroughly for about 30 seconds using a plate mixer(Be careful not to splatter the contents of the wells)