The lower part of the cap remained free for the discharge of basidiospores, which were collected in a solution containing glycerol (16%) and 2-(N-morpholine) ethane sulfonic (MES) acid (0.195%) with shaking for a period of 18 h under laboratory conditions. Basidiospore suspensions were kept in liquid nitro gen until use. Before use, the co ncentra tion of the basidio spore suspensi ons was adjusted to 5 105 basidiospores