Numerous cancer research for chemotherapeutic potential of medicinal plants have been carried out in an effort to discover new therapeutic agents that lack the toxic effects associated with current therapeutic agents.
Traditional medicine is commonly used as an alternative treatment for cancer by Thai people [10].
Several Thai traditional folklores have been shown to possess anticancer activities in various human cancerous cell lines with some promising candidates [11, 12].
In the present study, the ethanolic extracts of a total of 28 plants and 5 recipes used in Thai folklore medicine were investigated for their cytotoxic activity in vitro against three human cancerous cell lines, i.e., CL-6 (cholangiocarcinoma), Hep-2 (laryngeal carcinoma), HepG2 (hepatocarcinoma), and HRE (renal epithelial cells).
To our knowledge, this is the first study that focused on the investigation of cytotoxic activity of Thai folklore against cholangiocarcinoma.