Data were analyzed as a 4 × 4
Latin square design using the GLM
procedure of SAS (SAS Institute Inc.,
Cary, NC). The model included the
fixed effects of treatment and period,
the random effect of fermenter,
and the residual error. Least squares
means and SEM are reported for all
data. Significance was declared at P
< 0.05 and trends at P < 0.10.
True digestibility values of DM and
OM were defined as nutrient intake
minus nutrient effluent flow divided
by nutrient intake, with the effluent
corrected for buffer and microbial DM
and OM. Apparent digestibility values
of DM, OM, CP, and NDF were defined
as nutrient intake minus nutrient
effluent flow divided by nutrient
intake, with the effluent corrected for
buffer DM.