The absolute RGB values recorded in this study were likely to have been greatly impacted by the red colour of the tanks used for the 6 week trial. This can also influence the ability of animals to respond to exposure to black or white substrates. The increase in RGB values in most groups over time may indicate that, regardless of the treatment, the animals continued to adapt to their background over time. Tanks of another colour, such as mid-grey, may provide a better intermediate
colourin future work. Nonetheless,the effects of this study remain clear.