At sacrifice, the left lung was fixed for histomorphometric analysis
(median inter-wall distance, MIWD), whilst bronchoalveolar lavage fluid (BALF) was collected from the right lung. Cytological
analysis of BALF was performed and BALF was analysed for oxidant markers 8-iso-PGF2a, uric acid (UA), reduced (AA) and
oxidised ascorbic acid (DHA) and reduced (GSH) and oxidised glutathione (GSSG). Cd-exposure induced a significant
increase of BALF macrophages and neutrophils. 8-iso-PGF2a, UA, GSH and GSSG were significantly increased at D2. At 5W
and 5W þ 2, AA and GSH were significantly lower in Cd-exposed rats, indicating antioxidant depletion. MIWD significantly
increased in all repeatedly Cd-exposed groups, suggesting development of pulmonary emphysema. 8-iso-PGF2a and UA were
positively correlated with macrophage and neutrophil counts. GSH, GSSG and 8-iso-PGF2a were negatively correlated with
MIWD, indicating that Cd-induced emphysema could be associated with pulmonary oxidative stress.
At sacrifice, the left lung was fixed for histomorphometric analysis(median inter-wall distance, MIWD), whilst bronchoalveolar lavage fluid (BALF) was collected from the right lung. Cytologicalanalysis of BALF was performed and BALF was analysed for oxidant markers 8-iso-PGF2a, uric acid (UA), reduced (AA) andoxidised ascorbic acid (DHA) and reduced (GSH) and oxidised glutathione (GSSG). Cd-exposure induced a significantincrease of BALF macrophages and neutrophils. 8-iso-PGF2a, UA, GSH and GSSG were significantly increased at D2. At 5Wand 5W þ 2, AA and GSH were significantly lower in Cd-exposed rats, indicating antioxidant depletion. MIWD significantlyincreased in all repeatedly Cd-exposed groups, suggesting development of pulmonary emphysema. 8-iso-PGF2a and UA werepositively correlated with macrophage and neutrophil counts. GSH, GSSG and 8-iso-PGF2a were negatively correlated withMIWD, indicating that Cd-induced emphysema could be associated with pulmonary oxidative stress.
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