The moisture of the samples was adjusted to 70 g/100 g wet
basis by the addition of distilled water. The DSC (DSC 1, Mettler
Toledo (Thailand), Bangkok, Thailand) equipped with a refrigerated
cooler was used. The hydrated samples were weighed (25±5 mg)
into aluminium DSC pans (120mL) and hermetically sealed. The DSC
analysis was run by scanning from 25 to 120 C, ramping at 10 C/
min and a hermetically sealed empty pan was used as a reference.
Nitrogen was used as a purging gas. The software used for the
analysis of the resulting thermograms was Star
e
software (ver. 9.20,
Mettler Toledo). The onset temperature (To), peak temperature (Tp),
conclusion temperature (Tc) and transition enthalpy (DH)were
determined.