The mixture was treated with 20 ml of distilled
water and shaken. One ml of starch solution
(1.5% w/v) was added as an indicator. The peroxide value
(PV) was determined by titrating iodine liberated
from potassium iodide with sodium thiosulphate solution.
The PV was defined as the reactive oxygen content,
expressed as millimoles of free iodine per kg of lipid.