Three treatments were performed on individual 1 cm2 sections of each t-shirt.
T-shirt samples were added to plastic flasks containing either 150 ml deionised water (RW) or 70% ethanol (RE). The flasks were shaken for 24 h at 100 rpm (LuckhamR100/TW) before the t-shirt was removed, dried, and stored. The solution was then centrifuged (1200rpm), washed with deionised water, and the remaining supernatant discarded. The solution was prepared for diatom analysis as detailed below using known dilutions.
A third t-shirt sample for each location was treated by H2O2 digestion in a water bath. 20 ml of H2O2 (30%) was added to a test tube containing the t-shirt sample and heated in a water bath for 3h at 70C. The t-shirt was then removed and stored in distilled water. The solution was processed as above.
At all stages of sample collection and preparation for analysis, appropriate measures were taken in order to ensure contamina- tion between samples was avoided, in line with standard forensic procedures
Three treatments were performed on individual 1 cm2 sections of each t-shirt.
T-shirt samples were added to plastic flasks containing either 150 ml deionised water (RW) or 70% ethanol (RE). The flasks were shaken for 24 h at 100 rpm (LuckhamR100/TW) before the t-shirt was removed, dried, and stored. The solution was then centrifuged (1200rpm), washed with deionised water, and the remaining supernatant discarded. The solution was prepared for diatom analysis as detailed below using known dilutions.
A third t-shirt sample for each location was treated by H2O2 digestion in a water bath. 20 ml of H2O2 (30%) was added to a test tube containing the t-shirt sample and heated in a water bath for 3h at 70C. The t-shirt was then removed and stored in distilled water. The solution was processed as above.
At all stages of sample collection and preparation for analysis, appropriate measures were taken in order to ensure contamina- tion between samples was avoided, in line with standard forensic procedures
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