expressed as mg of rutin equivalents (RE)/g and as mg of naringin
equivalents (NE)/g of extract respectively.
2.5. Glucose tolerance test
The oral glucose tolerance test (Jain et al., 2010) was performed
in overnight fasted (18 h) normal rats. Rats were divided into ten
groups, each consisting of five rats. Group I was administered
with 0.9% w/v saline; group II received metformin (50 mg/kg i.p.);
Group III–X received CCM extract and its fractions in dose range
of 200 and 400 mg/kg per oral. Glucose (3 g/kg) was fed 30 min
after the administration of test samples. Blood was withdrawn
from tail-tip at 0, 30, 60 and 120 min of glucose administration and
glucose levels were estimated using glucose oxidase–peroxidase
reactive strips and a glucometer (Accuchek, Roche Diagnostics,
USA).