Due to this, this study tended to explore optimal operation strategy of PHA production using indigenous dye-decolorizing bacterium A. hydrophila NIU01 [10] for wastewater treatment and materials recycling and reuses afterwards. Recently, prior study [11] also showed the promising capability of PHA synthesis for A. hydrophila in the presence of decolorized intermediate(s) for wastewater decolorization. However, optimal strategy of operation to cellular capability of PHA synthesis was remained open to be explored for promising feasibility to practical applications. Thus, indigenous dye-decolorizing bacterium A. hydrophila NIU01 was selected to explore whether using coconut oil-bearing MR medium could significantly stimulate synthetic capabilities of PHAs.