Trails of the damaged cells caused by the larvae that migrated
through the monolayers were left. These trails were documented
by nuclear staining with PI (Figure 4B). In addition, the dead cells
were recognized by rabbit immune sera (Figure 4C). In contrast,
when the monolayers were inoculated with the non-activated
larvae, the larvae did not invade the cells and these cells were not
recognized by rabbit immune sera (Figure 4D).