RESULTS
Species-specific quantitative real-time PCR. The 5 nuclease
assay for detection of the genus Lactobacillus detected all
Lactobacillus species tested, but no other closely related genera
such as Enterococcus or Propionibacterium. The duplex 5
nuclease assays for the detection of the different Lactobacillus
species were specific as tested with the other (lactobacilli)
strains.