was 2m long with a 5mm OD and 2 mm ID and contained Porapak Q 100 that had a mesh range from 80–100.
Thecarrier gas was N2 , and the analysis was carried out at a carrier gas flow rate of 30 mL.minwith the injector,
column, and detector temperatures at 120, 90, and 120 ºC, respectively.
The pH of the anaerobic slurry (sludge) was measured using a digital pH meter, which had an accuracy of ±0.1 pH unit. Phosphate was analyzed by the molybdenum blue method [16]. Molybdenum acid ammonium solution, 2.0 ml, and an L-ascorbic acid solution, 1.0 ml, were added to the sample solution. After 15 min, the absorbance at a wavelength of 700 nm with UV–visible recording spectrophotometer (UVmini-1240 SHIMADZU) using 10 mm matched quartz cells.
The kinetic data obtained from all assays were checked for the fitness of modified Gompertz equation [17, 18].
The modified Gompertz equation, that gives cumulative biogas production from batch digesters assuming that biogas
production, is a function of bacterial growth.
The modified Gompertz equation is given by (Eq 1)
was 2m long with a 5mm OD and 2 mm ID and contained Porapak Q 100 that had a mesh range from 80–100. Thecarrier gas was N2 , and the analysis was carried out at a carrier gas flow rate of 30 mL.minwith the injector,column, and detector temperatures at 120, 90, and 120 ºC, respectively. The pH of the anaerobic slurry (sludge) was measured using a digital pH meter, which had an accuracy of ±0.1 pH unit. Phosphate was analyzed by the molybdenum blue method [16]. Molybdenum acid ammonium solution, 2.0 ml, and an L-ascorbic acid solution, 1.0 ml, were added to the sample solution. After 15 min, the absorbance at a wavelength of 700 nm with UV–visible recording spectrophotometer (UVmini-1240 SHIMADZU) using 10 mm matched quartz cells.The kinetic data obtained from all assays were checked for the fitness of modified Gompertz equation [17, 18].The modified Gompertz equation, that gives cumulative biogas production from batch digesters assuming that biogasproduction, is a function of bacterial growth. The modified Gompertz equation is given by (Eq 1)
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