Triacylglycerol composition
Triacylglycerol composition was measured based on AOCS CE 5-
86 (2004) methodology, determined by an Agilent capillary gas
chromatography system, Series 6850, with flame ionisation detector
(FID), and an Agilent DB-17 capillary column (50% phenylmethylpolysiloxane;
length 15 m, internal diameter 0.25 mm and
0.15 lm film thickness). Flow rate was 1.0 ml/min, linear velocity
of 40 cm/s, with a detector temperature of 375 C, injector temperature
of 360 C, oven temperature from 250 to 350 C (5 C/min),
followed by 350 C for 20 min, carrier gas of helium; injected volume
of 1.0 ll with 1:100 split. Retention times were determined
comparing with commercials standards, samples and others vegetable
oils (palm oil, soybean oil, and palm kernel oil) previously
indentified and quantified in our laboratory. All fractions of triacylglycerols
in palm oil were quantified based on relative peak area, in
duplicate. The results were compared with those obtained following
the procedure of Antoniosi Filho, Mendes, and Lanças (1995)
and with a literature compilation (Andrikopoulos, 2002).