All
the determination factors of equations of calibration
straight lines (R2) were greater than 0.99. The precision
of the method was calculated by comparing the determined
quantity of the substance with the quantity of
substance calculated based on statistical analysis using
the program ORIGIN 7 (Standard/Professional versions).
For all control samples, differences between experimental
samples and standards were never statistically significant
(P < 0.05). The accuracy (percentage deviation of
the concentration determined relative to the calculated
concentration) and the precision (standard deviation/
coefficient of variation) of methods always fell within
accepted ranges (± 15% of the mean).
The sensitivity of the method was calculated using 2
μg/mL of QE and 1 μg/mL of GA. In both instances,
when the accuracy and precision framed in admitted
limits in such determinations added up to the quantification
limit (± 20%).
Phytochemicals and in particular phenolic acids and
flavonoids compounds present in C. officinalis flowers
may be partially responsible for these health benefits
through their antioxidants activity. In the present study,
the main goals consist in isolating total flavonoid and
polyphenolic compounds by spectrophotometric assays
in the ultraviolet-visible. To distinguish rapidly between
various samples, flavonoids were extracted from flowers
by immersing (without crushing the tissue) in solvents
(HPLC method, we investigation for a patent
RO122836-B1).