In this study, the determination of total proteins present in WSE
was performed using the Bradford colorimetric method. In the past
it has been proven that the interference of carbohydrates present
in the sample matrix significantly deviates the actual absorbance
of the proteins, resulting in less accurate results if not primarily
removed (Banik, Pal, Ghorai, Chowdhury, & Khowala, 2009). Since
the primary aim of these preliminary studies was to observe the
hydrothermal degradation of proteins in SubCW and not quantify
the exact amount of proteins in extract, carbohydrates present in
WSE were not primarily precipitated and were analysed directly
using the above mentioned method.
In this study, the determination of total proteins present in WSEwas performed using the Bradford colorimetric method. In the pastit has been proven that the interference of carbohydrates presentin the sample matrix significantly deviates the actual absorbanceof the proteins, resulting in less accurate results if not primarilyremoved (Banik, Pal, Ghorai, Chowdhury, & Khowala, 2009). Sincethe primary aim of these preliminary studies was to observe thehydrothermal degradation of proteins in SubCW and not quantifythe exact amount of proteins in extract, carbohydrates present inWSE were not primarily precipitated and were analysed directlyusing the above mentioned method.
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